Addgene - PKC gamma DN Plasmid Data

Plasmid 21239: PKC gamma DN

Gene/insert name:
PKC gamma
Alt name:
protein kinase C, gamma
Insert size:
2088
Species:
M. musculus (mouse)
Entrez Gene:
Prkcg (PKCgamma, Pkcc, Prkcc)
Fusion protein or tag:
HA
Terminal:
C terminal on backbone
Mutation:
K380R
Vector backbone:
pHACE
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
SP6
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Dr. R.M. Bell
Sequence: View sequences (2)
Principal Investigator:
Bernard Weinstein
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Roles of specific isoforms of protein kinase C in the transcriptional control of cyclin D1 and related genes. Soh et al (J Biol Chem. 2003 Sep 5. 278(36):34709-16. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21239" in your Materials and Methods section.