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Plasmid 21282: zWnt5b
  • Wnt5b

  • 1091

  • D. rerio (zebrafish)

  • wnt5b (CH211-281K12.10-001, CHUNP6928, id:ibd5111, ppt, wnt-5, wnt5, wnt[b], wu:fk85g06)

  • pBluescript
    (Search Vector Database)

  • Stratagene

  • 3000

  • EcoRI

  • No

  • EcoRI

  • No

  • T7 List of Sequencing Primers

  • T3

  • Ampicillin

  • DH5alpha

  • 37

  • High Copy

  • From Phil Inghams' lab, cloned by P. Blader.

  • View sequences (2)
  • Randall Moon

  • UBMTA

Comments: 

Zebrafish wnt5 (pipetail) cDNA for antisense probe. Full-length ORF plus some (exact sequence not known, likely genbank NM_130937) 5' and 3' UTRs, 1.75kb total insert. Cloned into EcoRI site of pBluescript (there is also an internal EcoRI!). From Phil Inghams' lab, cloned by P. Blader.

Used to be called wnt5 and consider by many people to be wnt5a. however, after Gilbert cloned the real wnt5a, it is now known as wnt5b.
For antisense zWnt5b: cut w/ XbaI, transcribe w/ T3.

Addgene has sequenced a portion of this plasmid for verification. Full plasmid sequence is available only if provided by the depositing laboratory.

Article: Distinct Wnt signaling pathways have opposing roles in appendage regeneration. Stoick-Cooper et al (Development. 2007 Feb . 134(3):479-89. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21282" in your Materials and Methods section.

Price: US $65

Available to academic and non-profits only