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zWnt5b
(Plasmid #21282)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    Wnt5b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1091
  • Entrez Gene
    wnt5b (a.k.a. CH211-281K12.10-001, CHUNP6928, id:ibd5111, ppt, wnt-5, wnt5, wnt[b], wu:fk85g06)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From Phil Inghams' lab, cloned by P. Blader.
  • Terms and Licenses

Depositor Comments

Zebrafish wnt5 (pipetail) cDNA for antisense probe. Full-length ORF plus some (exact sequence not known, likely genbank NM_130937) 5' and 3' UTRs, 1.75kb total insert. Cloned into EcoRI site of pBluescript (there is also an internal EcoRI!). From Phil Inghams' lab, cloned by P. Blader.

Used to be called wnt5 and consider by many people to be wnt5a. however, after Gilbert cloned the real wnt5a, it is now known as wnt5b.
For antisense zWnt5b: cut w/ XbaI, transcribe w/ T3.

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    zWnt5b was a gift from Randall Moon (Addgene plasmid # 21282)
  • For your References section:

    Distinct Wnt signaling pathways have opposing roles in appendage regeneration. Stoick-Cooper CL, Weidinger G, Riehle KJ, Hubbert C, Major MB, Fausto N, Moon RT. Development. 2007 Feb . 134(3):479-89. 10.1242/dev.001123 PubMed 17185322