Addgene - zWnt5b Plasmid Data

Plasmid 21282: zWnt5b

Gene/insert name:
Wnt5b
Insert size:
1091
Species:
D. rerio (zebrafish)
Entrez Gene:
wnt5b (CH211-281K12.10-001, CHUNP6928, id:ibd5111, ppt, wnt-5, wnt5, wnt[b], wu:fk85g06)
Vector backbone:
pBluescript
(Search Vector Database)
Backbone manufacturer:
Stratagene
Backbone size w/o insert (bp):
3000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
T3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
From Phil Inghams' lab, cloned by P. Blader.
Sequence: View sequences (2)
Principal Investigator:
Randall Moon
Terms and Licenses:
MTA

Comments:

Zebrafish wnt5 (pipetail) cDNA for antisense probe. Full-length ORF plus some (exact sequence not known, likely genbank NM_130937) 5' and 3' UTRs, 1.75kb total insert. Cloned into EcoRI site of pBluescript (there is also an internal EcoRI!). From Phil Inghams' lab, cloned by P. Blader.

Used to be called wnt5 and consider by many people to be wnt5a. however, after Gilbert cloned the real wnt5a, it is now known as wnt5b.
For antisense zWnt5b: cut w/ XbaI, transcribe w/ T3.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Distinct Wnt signaling pathways have opposing roles in appendage regeneration. Stoick-Cooper et al (Development. 2007 Feb . 134(3):479-89. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21282" in your Materials and Methods section.