zWnt5b
(Plasmid #21282)

• Depositing Lab: Randall Moon
• Publication: Stoick-Cooper et al Development. 2007 Feb . 134(3):479-89.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3000

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Wnt5b
• Species: D. rerio (zebrafish)
• Insert Size (bp): 1091
• Entrez Gene: wnt5b (a.k.a. CHUNP6928, id:ibd5111, pipetail, ppt, wnt-5, wnt5, wnt[b], wu:fk85g06)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: From Phil Inghams' lab, cloned by P. Blader.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Zebrafish wnt5 (pipetail) cDNA for antisense probe. Full-length ORF plus some (exact sequence not known, likely genbank NM_130937) 5' and 3' UTRs, 1.75kb total insert. Cloned into EcoRI site of pBluescript (there is also an internal EcoRI!). From Phil Inghams' lab, cloned by P. Blader.

Used to be called wnt5 and consider by many people to be wnt5a. however, after Gilbert cloned the real wnt5a, it is now known as wnt5b.
For antisense zWnt5b: cut w/ XbaI, transcribe w/ T3.