pCS2-MT mouse Axin (Axin MTFu1)
(Plasmid #21287)

• Depositing Lab: Frank Costantini
• Publication: Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Backbone

• Vector backbone: pCS2+MT
• Backbone size w/o insert (bp): 4300
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Axin
• Species: M. musculus (mouse)
• Entrez Gene: Axin1 (a.k.a. Axin, Fu, Kb, Ki, fused, kinky, knobbly)
• Tag / Fusion Protein:
  • Myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Addgene Notes:
  • Sequencing result from requesting lab.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry