Addgene - pBabe puro MTH1 Plasmid Data

Plasmid 21295: pBabe puro MTH1

Gene/insert name:
MTH1
Species:
H. sapiens (human)
Entrez Gene:
NUDT1 (MTH1)
Vector backbone:
pBABE-puro
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#1764)
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
5169
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pBABE 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
Dr. Yusaku Nakabeppu provided pcDEB.MTH1
Sequence: View sequences (1)
Principal Investigator:
Bob Weinberg
Terms and Licenses:
MTA

Comments:

The pBabe.puro MTH1 overexpression construct was created by amplifying the MTH1 cDNA from pcDEB.MTH1 (which expresses full-length wild type human MTH1) using PCR
primers to add BamHI and EcoRI cloning sites to the 5' and 3'
ends respectively, and ligating the resulting fragment into the appropriately digested retroviral pBABE puro vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Continuous elimination of oxidized nucleotides is necessary to prevent rapid onset of cellular senescence. Rai et al (Proc Natl Acad Sci U S A. 2009 Jan 6. 106(1):169-74. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21295" in your Materials and Methods section.