Addgene - PL-SIN-EF1α-EGFP Plasmid Data

Plasmid 21320: PL-SIN-EF1α-EGFP

Gene/insert name:
Enhanced Green Fluorescence Protein
Alt name:
EGFP
Insert size:
720
Vector backbone:
PL-SIN lentiviral vector
(Search Vector Database)
Backbone manufacturer:
Buzina et al., PLoS Genetics, 2008
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
6881
Promoter:
EF1α
Cloning method:
Unknown
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (2)
Principal Investigator:
James Ellis
Terms and Licenses:
MTA

Comments:

EGFP driven by ubiquitous EF1α promoter. PL-SIN lentiviral vector (Buzina et al., PLoS Genetics, 2008) requires Tat cDNA for lentivirus production, as well as other packaging cDNAs (Gag/pol, Rev, and VSV-G).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Isolation of human iPS cells using EOS lentiviral vectors to select for pluripotency. Hotta et al (Nat Methods. 2009 May . 6(5):370-6. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21320" in your Materials and Methods section.