Addgene - pLKO mouse shRNA 1 raptor Plasmid Data

Plasmid 21339: pLKO mouse shRNA 1 raptor

Gene/insert name:
raptor shRNA 1
Alt name:
raptor
Insert size:
60
Species:
M. musculus (mouse)
GenBank ID:
NM_028898
Entrez Gene:
Rptor (Rap, Raptor, mKIAA1303, 4932417H02Rik, RP24-225K18.1)
Vector backbone:
pLKO.1
(Search Vector Database)
Backbone manufacturer:
Available from Addgene
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7032
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL10 Gold
Growth temperature (℃):
37
Growth instructions:
XL10 Gold Ultracompetent Cells from Stratagene or nStb13. Grow in SOC media at 37oC
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Supplemental document:
Addgene's pLKO.1 protocol
Supplemental document:
RNAi Consortium clone information
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

mouse raptor shRNA 1.

shRNA oligo sequence is 5'- CCGGCCTCATCGTCAAGTCCTTCAACTCGAGTTGAAGGACTTGACGATGAGGTTTTTG -3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. Thoreen et al (J Biol Chem. 2009 Mar 20. 284(12):8023-32. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21339" in your Materials and Methods section.