pLKO mouse shRNA 2 raptor
(Plasmid #21340)

• Depositing Lab: David Sabatini
• Publication: Peterson et al Cell. 2009 May 13. ():.

Backbone

• Vector backbone: pLKO.1
• Backbone manufacturer: Available from Addgene
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL10 Gold
• Growth instructions: XL10 Gold Ultracompetent Cells from Stratagene or nStb13. Grow in SOC media at 37oC
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: raptor shRNA 2
• Alt name: raptor
• gRNA/shRNA sequence: CCGGGCCCGAGTCTGTGAATGTAATCTCGAGATTACATTCACAGACTCGGGCTTTTTG
• Species: M. musculus (mouse)
• Insert Size (bp): 60
• GenBank ID: NM_028898
• Entrez Gene: Rptor (a.k.a. 4932417H02Rik, Rap, Raptor, mKIAA1303)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: LKO.1 5'

Resource Information

• References:
  • Addgene's pLKO.1 protocol
  • RNAi Consortium clone information
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

mouse raptor shRNA 2

shRNA oligo sequence is 5'- CCGGGCCCGAGTCTGTGAATGTAATCTCGAGATTACATTCACAGACTCGGGCTTTTTG -3'