Addgene - pLKO mouse shRNA 2 raptor Plasmid Data

Plasmid 21340: pLKO mouse shRNA 2 raptor

Gene/insert name:
raptor shRNA 2
Alt name:
raptor
Insert size:
60
Species:
M. musculus (mouse)
GenBank ID:
NM_028898
Vector backbone:
pLKO.1
(Search Vector Database)
Backbone manufacturer:
Available from Addgene
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size w/o insert (bp):
7032
Cloning site 5':
AgeI
Site destroyed during cloning:
Unknown
Cloning site 3':
EcoRI
Site destroyed during cloning:
Unknown
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL10 Gold
Growth temperature (℃):
37
Growth instructions:
XL10 Gold Ultracompetent Cells from Stratagene or nStb13. Grow in SOC media at 37oC
High or low copy:
Unknown
Selectable markers:
Puromycin
Sequence: View sequences (1)
Supplemental document:
Addgene's pLKO.1 protocol
Supplemental document:
RNAi Consortium clone information
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

mouse raptor shRNA 2

shRNA oligo sequence is 5'- CCGGGCCCGAGTCTGTGAATGTAATCTCGAGATTACATTCACAGACTCGGGCTTTTTG -3'

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: DEPTOR Is an mTOR Inhibitor Frequently Overexpressed in Multiple Myeloma Cells and Required for Their Survival. Peterson et al (Cell. 2009 May 13. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21340" in your Materials and Methods section.