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WT (REP10)
(Plasmid #21368)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21368 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pREP10
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 8500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5a in dYT at 37oC
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autosomal dominant polycystic kidney disease type I
  • Alt name
    PKD1 wild-type
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    14000
  • GenBank ID
    L33243
  • Entrez Gene
    PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (destroyed during cloning)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WT (REP10) was a gift from Gregory Germino (Addgene plasmid # 21368 ; http://n2t.net/addgene:21368 ; RRID:Addgene_21368)
  • For your References section:

    Cleavage of polycystin-1 requires the receptor for egg jelly domain and is disrupted by human autosomal-dominant polycystic kidney disease 1-associated mutations. Qian F, Boletta A, Bhunia AK, Xu H, Liu L, Ahrabi AK, Watnick TJ, Zhou F, Germino GG. Proc Natl Acad Sci U S A. 2002 Dec 24. 99(26):16981-6. 10.1073/pnas.252484899 PubMed 12482949