M-PKD2 (OF2-3)
(Plasmid #21370)

• Depositing Lab: Gregory Germino
• Publication: Hanaoka et al Nature. . 408(6815):990-4.

Backbone

• Vector backbone: pCS2+MT
• Backbone size w/o insert (bp): 4352
• Vector type: Mammalian Expression
• Selectable markers: none

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5a in dYT at 37oC
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: autosomal dominant polycystic kidney disease type II
• Alt name: PKD2 wild-type
• Species: H. sapiens (human)
• Insert Size (bp): 3092
• Mutation: wild-type*
• GenBank ID: NM_000297
• Entrez Gene: PKD2 (a.k.a. APKD2, PC2, PKD4, Pc-2, TRPP2)
• Tag / Fusion Protein:
  • 6xMyc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: StuI (destroyed during cloning)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

*Note that this construct has a rare SNP (rs1131408) at position 4:88967820 that results in a missense change (G449V). The polymorphism is not known to affect function.