Addgene - pCM5.49 Plasmid Data

Plasmid 21391: pCM5.49

Gene/insert name:
pal-1 3'utr in pDONRP2RP3
Insert size:
538
Species:
C. elegans (nematode)
Vector backbone:
pDONRP2RP3
(Search Vector Database)
Vector type:
Gateway
Backbone size w/o insert (bp):
2641
Cloning method:
Gateway Cloning
Cloning site 5':
attP2R
Site destroyed during cloning:
Yes
Cloning site 3':
attP3
Site destroyed during cloning:
Yes
5' sequencing primer:
M13Forward List of Sequencing Primers
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Principal Investigator:
Geraldine Seydoux
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: 3' UTRs Are the Primary Regulators of Gene Expression in the C. elegans Germline. Merritt et al (Curr Biol. 2008 Sep 23. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21391" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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