Addgene - pEGFP-N1-FIH1 Plasmid Data

Plasmid 21403: pEGFP-N1-FIH1

Gene/insert name:
HIF1AN
Alt name:
FIH1
Insert size:
1050
Species:
H. sapiens (human)
Entrez Gene:
HIF1AN (FIH1)
Fusion protein or tag:
EGFP
Terminal:
C terminal on backbone
Vector backbone:
pEGFP-N1
(Search Vector Database)
Backbone manufacturer:
Clontech
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
SacII
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
EGFP-N
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Eric Metzen
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Intracellular localisation of human HIF-1 alpha hydroxylases: implications for oxygen sensing. Metzen et al (J Cell Sci. 2003 Apr 1. 116(Pt 7):1319-26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21403" in your Materials and Methods section.