Addgene - pJA252 Plasmid Data

Plasmid 21512: pJA252

Gene/insert name:
pmex-5 (w/o ATG)
Insert size:
489
Species:
C. elegans (nematode)
Vector backbone:
pDONR P4-P1R
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
MultiSite Gateway
Backbone size w/o insert (bp):
2643
Cloning method:
Gateway Cloning
Cloning site 5':
attP4
Site destroyed during cloning:
Yes
Cloning site 3':
attP1R
Site destroyed during cloning:
Yes
5' sequencing primer:
M13F List of Sequencing Primers
3' sequencing primer:
M13R/ T7
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5a
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Map:
View map
Principal Investigator:
Julie Ahringer
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: MosSCI and gateway compatible plasmid toolkit for constitutive and inducible expression of transgenes in the C. elegans germline. Zeiser et al (PLoS One. 2011;6(5):e20082. Epub 2011 May 26. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21512" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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