|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21523||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7000
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Insert Size (bp)1500
Entrez GeneTNRC6A (a.k.a. CAGH26, GW1, GW182, TNRC6)
/ Fusion Protein
- GST (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Stu I (destroyed during cloning)
- 5′ sequencing primer CAGCAAGTATATAGCATGGC (Common Sequencing Primers)
Terms and Licenses
In our JCS paper (Li et al. J Cell Sci. 2008 Dec 15;121(Pt 24):4134-44), a new isoform of GW182 was identified with an extra N-terminal 253-amino-acid. That is why the name of these two clones changed from the original GW182D1a (aa1-250) and GW182D1(aa1-498) to GW1D1a(aa254-503) and GW1D1(aa254-751), respectively.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:GST-GW1D1(aa254-751) was a gift from Edward Chan (Addgene plasmid # 21523)
For your References section:The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing. Lian SL, Li S, Abadal GX, Pauley BA, Fritzler MJ, Chan EK. RNA. 2009 May . 15(5):804-13. 10.1261/rna.1229409 PubMed 19324964