Addgene - pLenti X2 Zeo DEST (749-3) Plasmid Data

Plasmid 21562: pLenti X2 Zeo DEST (749-3)

Gene/insert name:
No insert
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral, RNAi
Backbone size (bp):
6909
5' sequencing primer:
n/a List of Sequencing Primers
Bacterial resistance(s)
Chloramphenicol and Bleocin (Zeocin)
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1, 37oC, low salt LB
High or low copy:
High Copy
Selectable markers:
Zeocin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells Campeau et al (PLoS One. 2009 Aug 6;4(8):e6529. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21562" in your Materials and Methods section.