Addgene - pIE2-AD Plasmid Data

Plasmid 21603: pIE2-AD

Gene/insert name:
NF-kappa-B p65 subunit
Alt name:
p65
Insert size:
681
Species:
M. musculus (mouse)
GenBank ID:
NP_033071
Entrez Gene:
Rela (p65)
Fusion protein or tag:
NLS
Terminal:
N terminal on backbone
Fusion protein or tag:
Flag
Terminal:
N terminal on backbone
Fusion protein or tag:
Gateway destination cassette
Terminal:
C terminal on backbone
Vector backbone:
pIZT
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Insect Expression ; Gateway Destination Vector
Backbone size w/o insert (bp):
4635
Cloning method:
Gateway Cloning
5' sequencing primer:
TTATCGCGCCTATAAATACAGCCCGCAAC List of Sequencing Primers
3' sequencing primer:
CACGCGCTTGAAAGGAGTGTGTAAATGGAC
Bacterial resistance(s)
Tetracycline
Growth strain(s)
DB3.1
Growth temperature (℃):
37
Growth instructions:
DB3.1 (Invitrogen) 37°C
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Takahiro Kusakabe
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Analysis of protein interactions with two-hybrid system in cultured insect cells. Mon et al (Anal Biochem. 2009 May 26. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21603" in your Materials and Methods section.

Price: $65.00

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