pLLNeo-Oct3
(Plasmid #21618)

• Depositing Lab: David Garbers
• Publication: Dann et al Stem Cells. 2008 Nov . 26(11):2928-37.

Backbone

• Vector backbone: pLLU2G
• Backbone size w/o insert (bp): 7861
• Vector type: Lentiviral, RNAi
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Oct3 shRNA
• Alt name: Oct4, Pou5f1
• Insert Size (bp): 54

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: BGH-rev
• 3′ sequencing primer: mPGK-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Oct3 shRNA sequence is - 5'-TTGTTTGAACCGTGTGAGGTGGAACTTCAAGAGAGTTCCACCTCACACGGTTCT

This is a 3rd generation lentiviral plasmid.

This plasmid worked for mouse and rat Oct4 knockdown, but it was not tested in human cells.