Addgene - pIS1 Cyclin D2 long-mut-miR15/16 Plasmid Data

Plasmid 21647: pIS1 Cyclin D2 long-mut-miR15/16

Gene/insert name:
Cyclin D2 long-mut-miR15/16
Alt name:
CM7
Alt name:
Cyclin D2
Insert size:
5300
Species:
H. sapiens (human)
Entrez Gene:
CCND2 (KIAK0002)
Fusion protein or tag:
Luciferase
Terminal:
N terminal on backbone
Mutation:
Mutant miR15/16 microRNA binding sites
Vector backbone:
pIS1
(Search Vector Database)
Backbone manufacturer:
Available from Addgene (#12179)
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4085
Cloning site 5':
Xba I
Site destroyed during cloning:
No
Cloning site 3':
Not I
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
EBV Reverse
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
David Bartel
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Long means that all functional alternative polyadenylation signals have been deleted.

Mut means that all microRNA binding sites for an indicated microRNA have been mutated.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Widespread shortening of 3'UTRs by alternative cleavage and polyadenylation activates oncogenes in cancer cells. Mayr et al (Cell. 2009 Aug 21. 138(4):673-84. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21647" in your Materials and Methods section.