Gene/insert name: Vacuolar Protein Sorting 35 homolog
Alt name: VPS35
Insert size: 2391
Species:
H. sapiens (human)
GenBank ID:
NM_018206
Entrez Gene:
VPS35
(MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672)
Fusion protein or tag: V5
Terminal: C terminal on backbone
Vector backbone:
pLenti6/V5/DEST
(Search Vector Database )
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Lentiviral ; Invitrogen Gateway Destination vector
Backbone size w/o insert (bp):
2391
Cloning method: Gateway Cloning
Cloning site 5':
att
Site destroyed during cloning:
No
Cloning site 3':
att
Site destroyed during cloning:
No
5' sequencing primer:
CMVF
List of Sequencing Primers
3' sequencing primer:
V5R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Invitrogen stbl3
High or low copy:
Unknown
Selectable markers:
Blasticidin
Sequence:
View sequences (1)
Principal Investigator:
Lynda Chin
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
21691" in your Materials and Methods section.