pRK5 FLAG DEPTOR (13×S/T->A)
(Plasmid #21702)

• Depositing Lab: David Sabatini
• Publication: Peterson et al Cell. 2009 May 13. ():.

Backbone

• Vector backbone: pRK5-FLAG
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: DEPTOR 13xA
• Alt name: DEPDC6
• Alt name: DEPTOR
• Species: H. sapiens (human)
• Insert Size (bp): 2350
• Mutation: Nonphosphorylatable mutant. 13xA. Also has N204S and S389N mutations (numbering according to associated publication)
• GenBank ID: NM_022783
• Entrez Gene: DEPTOR (a.k.a. DEP.6, DEPDC6, hDEPTOR)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SalI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

DEPTOR phosphorylation sites depicted in Author's Map link.