Addgene - pAc5.1B-EGFP-DmTral Plasmid Data

Plasmid 21722: pAc5.1B-EGFP-DmTral

Gene/insert name:
Trailer hitch
Alt name:
Tral
Insert size:
1959
Species:
D. melanogaster (fly)
GenBank ID:
NM_140328
Entrez Gene:
tral (Dmel_CG10686, BEST:GH08269, CG10686, DmLSm15, Dmel\CG10686, LSm15, Lsm15, Scd6p, TRAL, Tral, anon-EST:Posey139, anon-WO0172774.89, anon-WO02059370.24, p67, p70)
Fusion protein or tag:
EGFP
Terminal:
N terminal on backbone
Vector backbone:
pAc5.1B-EGFP
(Search Vector Database)
Backbone manufacturer:
Elisa Izaurralde
Vector type:
Drosophila expression
Backbone size w/o insert (bp):
6070
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
Ac5 forward primer site List of Sequencing Primers
3' sequencing primer:
BGH reverse priming site
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Dh5 alpha or XL1-blue
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Elisa Izaurralde
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: P-body formation is a consequence, not the cause, of RNA-mediated gene silencing. Eulalio et al (Mol Cell Biol. 2007 Jun . 27(11):3970-81. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21722" in your Materials and Methods section.