AIRAP 2: AIRAP(m1.9-T7)pGEX.KG
(Plasmid #21792)

• Depositing Lab: David Ron
• Publication: Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Backbone

• Vector backbone: pGEX.KG
• Backbone manufacturer: ATCC
• Backbone size w/o insert (bp): 5006
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): BL21
• Growth instructions: Strain should be BL21
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AIRAP
• Species: M. musculus (mouse)
• Insert Size (bp): 1250
• GenBank ID: AF224494
• Entrez Gene: Zfand2a (a.k.a. Airap, AA407930, AU016206)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: pGEX-5
• 3′ sequencing primer: pGEX-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We DO NOT have a good record for how this plasmid was constructed.
The best guess is that Jin Liu used the m1.9Bam.1S primer and T7
to PCR the CDS + 3'UTR from m1.9pBS and ligated the BamH1-Xba1
fragment in frame with GST in pGEX.KG