TLS 5:hTLS-CHOP.pCDNA1
(Plasmid #21831)

• Depositing Lab: David Ron
• Publication: Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Backbone

• Vector backbone: pCDNA1
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: This plasmid must be propagated in P3-containing host strain such as DH10B/P3 under combined amp (25mcg/ml)/tet (10mcg/ml) selection pressure.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TLS-CHOP
• Species: H. sapiens (human)
• Insert Size (bp): 1700
• Entrez Gene: FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Human TLS-CHOP full-length cDNA in pCDNA1. Also known as hLPS41.pCDNA1.