|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21870||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8723
Vector typeBacterial Expression
Growth in Bacteria
Growth instructionsGrow in 30ug/mL Kanamycin. This is a low copy number plasmid and therefore we recommend that minipreps be performed from a 10 ml culture.
Copy numberLow Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer lac promoter
- 3′ sequencing primer M13 forward20 (Common Sequencing Primers)
This plasmid is used to construct OPEN B2H selection strains(see Maeder et al., Mol Cel 2008, PMID 18657511). Clone DNA binding sites into this reporter plasmid.
Annotations for pKJ1712:
581-1492: p15A origin of replication / 1664-2415: C-terminal lacI gene fragment (for recombination) / 2862-3673: Kanamycin-resistance gene / 3987-4030: rrnB T1 terminator / 4162-4189: rrnB T2 terminator / 4319-4325: SapI site #1 / 4352-4358: SapI site #2 / 4381-4386: lac promoter -35 box / 4405-4410: lac promoter -10 box / 4417: lac promoter transcription start point / 4452-4454: HIS3 gene start codon / 5112-5114: HIS3 gene stop codon / 5162-5164: aadA gene start codon / 5951-5953: aadA gene stop codon / 5960-6576: lacZ gene fragment (for recombination) / 6833-6835: sacB stop codon (rev comp strand) / 6833-8254: sacB coding sequence (rev comp strand) / 8252-8254: sacB start codon (rev comp strand)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pKJ1712 was a gift from Keith Joung (Addgene plasmid # 21870 ; http://n2t.net/addgene:21870 ; RRID:Addgene_21870)