Addgene - pKJ1712 Plasmid Data

Plasmid 21870: pKJ1712

Gene/insert name:
None
Vector backbone:
pKJ1712
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size (bp):
8723
5' sequencing primer:
lac promoter List of Sequencing Primers
3' sequencing primer:
M13 forward20
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
Grow in 30ug/mL Kanamycin. This is a low copy number plasmid and therefore we recommend that minipreps be performed from a 10 ml culture.
High or low copy:
Low Copy
Sequence: View sequences (3)
Map:
View map
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

This plasmid is used to construct OPEN B2H selection strains(see Maeder et al., Mol Cel 2008, PMID 18657511). Clone DNA binding sites into this reporter plasmid.

Annotations for pKJ1712:

581-1492: p15A origin of replication / 1664-2415: C-terminal lacI gene fragment (for recombination) / 2862-3673: Kanamycin-resistance gene / 3987-4030: rrnB T1 terminator / 4162-4189: rrnB T2 terminator / 4319-4325: SapI site #1 / 4352-4358: SapI site #2 / 4381-4386: lac promoter -35 box / 4405-4410: lac promoter -10 box / 4417: lac promoter transcription start point / 4452-4454: HIS3 gene start codon / 5112-5114: HIS3 gene stop codon / 5162-5164: aadA gene start codon / 5951-5953: aadA gene stop codon / 5960-6576: lacZ gene fragment (for recombination) / 6833-6835: sacB stop codon (rev comp strand) / 6833-8254: sacB coding sequence (rev comp strand) / 8252-8254: sacB start codon (rev comp strand)

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21870" in your Materials and Methods section.