Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Addgene is operating at a reduced capacity.

  • Pending order? We will email to confirm that your organization can accept shipments.
  • Conducting coronavirus or COVID-19 research? Email us at [email protected] with your order or deposit number so we can prioritize it.

Learn more about our current shipping status and COVID-19 resources.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pKJ1712
(Plasmid #21870)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21870 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow in 30ug/mL Kanamycin. This is a low copy number plasmid and therefore we recommend that minipreps be performed from a 10 ml culture.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    None

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer lac promoter
  • 3′ sequencing primer M13 forward20
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

This plasmid is used to construct OPEN B2H selection strains(see Maeder et al., Mol Cel 2008, PMID 18657511). Clone DNA binding sites into this reporter plasmid.

Annotations for pKJ1712:

581-1492: p15A origin of replication / 1664-2415: C-terminal lacI gene fragment (for recombination) / 2862-3673: Kanamycin-resistance gene / 3987-4030: rrnB T1 terminator / 4162-4189: rrnB T2 terminator / 4319-4325: SapI site #1 / 4352-4358: SapI site #2 / 4381-4386: lac promoter -35 box / 4405-4410: lac promoter -10 box / 4417: lac promoter transcription start point / 4452-4454: HIS3 gene start codon / 5112-5114: HIS3 gene stop codon / 5162-5164: aadA gene start codon / 5951-5953: aadA gene stop codon / 5960-6576: lacZ gene fragment (for recombination) / 6833-6835: sacB stop codon (rev comp strand) / 6833-8254: sacB coding sequence (rev comp strand) / 8252-8254: sacB start codon (rev comp strand)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKJ1712 was a gift from Keith Joung (Addgene plasmid # 21870 ; http://n2t.net/addgene:21870 ; RRID:Addgene_21870)