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CHOP 4: Mouse CHOP-WT-9E10-pSRa
(Plasmid #21903)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 21903 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSRa
  • Backbone size w/o insert (bp) 4534
  • Vector type
    Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CHOP10
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    605
  • Entrez Gene
    Ddit3 (a.k.a. AltDDIT3, CHOP-10, CHOP10, chop, gadd153)
  • Tag / Fusion Protein
    • 9E10-myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site HindIII (unknown if destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer Neo-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pSRa and its associated helper plasmids are described in: Muller, AJ et al 1992 Mol Cell Biol 11:941, and helper plasmids were obtained from the lab of Dr. Owen Witte

NB: From Addgene's sequencing results, there is a 6bp insertion into the insert of CHOP4 that is not in the depositor's sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CHOP 4: Mouse CHOP-WT-9E10-pSRa was a gift from David Ron (Addgene plasmid # 21903 ; http://n2t.net/addgene:21903 ; RRID:Addgene_21903)