Addgene - pGEX-2TK-PTB Plasmid Data

Plasmid 21929: pGEX-2TK-PTB

Gene/insert name:
PTB
Alt name:
PTBP1
Insert size:
1600
Species:
H. sapiens (human)
Entrez Gene:
PTBP1 (HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
pGEX-2TK
(Search Vector Database)
Backbone manufacturer:
Amersham
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX-5 List of Sequencing Primers
3' sequencing primer:
pGEX-3
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Supplemental document:
Notes from Addgene (1)
Principal Investigator:
Phil Sharp
Terms and Licenses:
MTA

Comments:

F29L and H62L in Addgene seq result

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Garcia-Blanco et al (Genes Dev. 1989 Dec . 3(12A):1874-86. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 21929" in your Materials and Methods section.