pGEX-2TK-PTB
(Plasmid #21929)

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Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Sequence Information

Full plasmid sequence is available only if provided by the depositing laboratory.

Gene/Insert

  • Gene/Insert name
    PTB
  • Alt name
    PTBP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Entrez Gene
    PTBP1 (a.k.a. HNRNP-I, HNRNPI, HNRPI, PTB, PTB-1, PTB-T, PTB2, PTB3, PTB4, pPTB)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX-5
  • 3′ sequencing primer pGEX-3
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

F29L and H62L in Addgene seq result

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-2TK-PTB was a gift from Phil Sharp (Addgene plasmid # 21929)
  • For your References section:

    Identification and purification of a 62,000-dalton protein that binds specifically to the polypyrimidine tract of introns. Garcia-Blanco MA, Jamison SF, Sharp PA. Genes Dev. 1989 Dec . 3(12A):1874-86. 10.1101/gad.3.12a.1874 PubMed 2533575