Addgene - pCE-BiFC-VN173 Plasmid Data

Plasmid 22019: pCE-BiFC-VN173

Gene/insert name:
VN173
Alt name:
Venus(1-172)
Insert size:
522
Species:
Aequorea Victoria
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Mutation:
N/A
Vector backbone:
pPD4983
(Search Vector Database)
Backbone manufacturer:
Fire lab 1995 kit
Vector type:
Worm Expression
Backbone size w/o insert (bp):
3993
Cloning site 5':
KpnI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
DH5 alpha
High or low copy:
High Copy
Sequence: View sequences (3)
Principal Investigator:
Chang-Deng Hu
Terms and Licenses:
MTA

Comments:

Hiatt, S.M., Shyu, Y., Duren, H.M, and Hu, C.D. Bimolecular fluorescence complementation (BiFC) analysis of protein interactions in living C. elegans. Methods, 45:185-191 (2008)

Multicloning sites betwee Myc and VN173 are avaialble

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Visualization of protein interactions in living Caenorhabditis elegans using bimolecular fluorescence complementation analysis. Shyu et al (Nat Protoc. 2008 . 3(4):588-96. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22019" in your Materials and Methods section.