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pSTK120-mCMV-TK
(Plasmid #22054)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22054 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSTK120
  • Backbone size w/o insert (bp) 28750
  • Vector type
    Adenoviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    32C and 160 RPM
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCMV-TK-pA expression cassette
  • Alt name
    HSV1-TK
  • Alt name
    thymidine kinase
  • Alt name
    TK
  • Species
    Herpes Simplex Type 1
  • Insert Size (bp)
    3862

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EagI (not destroyed)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSTK120-mCMV-TK was a gift from Maria Castro (Addgene plasmid # 22054 ; http://n2t.net/addgene:22054 ; RRID:Addgene_22054)
  • For your References section:

    High-capacity adenovirus vector-mediated anti-glioma gene therapy in the presence of systemic antiadenovirus immunity. King GD, Muhammad AK, Xiong W, Kroeger KM, Puntel M, Larocque D, Palmer D, Ng P, Lowenstein PR, Castro MG. J Virol. 2008 May . 82(9):4680-4. 10.1128/JVI.00232-08 PubMed 18287240