Addgene - pWJPrP31 Plasmid Data

Plasmid 22105: pWJPrP31

Gene/insert name:
PrP exon 3 EcoR1 fragment
Insert size:
2200
Species:
M. musculus (mouse)
Mutation:
genomic EcoR1 fragment containing exon 3 of PrP
Vector backbone:
pBTIIKS(+)
(Search Vector Database)
Vector type:
construction of targeting vector
Backbone size w/o insert (bp):
2900
Cloning site 5':
EcoR1
Site destroyed during cloning:
No
Cloning site 3':
EcoR1
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
David Melton; Edinburgh, Scotland
Sequence: View sequences (2)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Spontaneous generation of prion infectivity in fatal familial insomnia knockin mice. Jackson et al (Neuron. 2009 Aug 27. 63(4):438-50. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22105" in your Materials and Methods section.