pACYC-FLAG-dN6-His
(Plasmid #22143)

• Depositing Lab: Robert Sauer
• Publication: Martin et al Nature. 2005 Oct 20. 437(7062):1115-20.

Backbone

• Vector backbone: pACYC.pET24cloning
• Backbone manufacturer: modifiedNovagen
• Backbone size w/o insert (bp): 3500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Bl21 RecA-, chloramphenical-resistant (35 ug/mL) in LB at 37 C
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Flag-clpXdeltaNLinkedHexamer-His6
• Alt name: linked hexamer
• Alt name: N-terminal Flag hexamer
• Alt name: C-terminal His hexamer
• Species: E. coli
• Insert Size (bp): 7164
• Tags / Fusion Proteins:
  • Flag (N terminal on insert)
  • His6 (C terminal on insert)
  • L20 Linkers

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: t7
• 3′ sequencing primer: t7rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr. Andreas Martin, UC Berkeley
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry