pLenti X2 Zeo/pTER shMDC1 (764-1)
(Plasmid #22194)

Available to Academic and Nonprofits Only

Backbone

  • Vector backbone
    p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
  • Backbone size w/o insert (bp) 5587
  • Vector type
    Lentiviral, RNAi
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin(Zeocin)
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Stbl3, 37oC
  • Copy number
    High Copy

Sequence Information

Gene/Insert

  • Gene/Insert name
    shMediator of DNA damage checkpoint 1
  • Alt name
    shMDC1
  • Insert Size (bp)
    58

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal I (not destroyed)
  • 3′ cloning site Xba I (not destroyed)
  • 5′ sequencing primer H1forw
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti X2 Zeo/pTER shMDC1 (764-1) was a gift from Eric Campeau (Addgene plasmid # 22194)