peCALWY-4
(Plasmid #22236)

• Depositing Lab: Maarten Merkx
• Publication: Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.

Backbone

• Vector backbone: pCMV (Clontech)
• Backbone size w/o insert (bp): 3900
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: E.coli nova blue LB culture medium 30 ug/ml kanamycin Culture overnight, 37 C/250 rpm
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: eCALWY-4 Zn(II) sensor
• Species: H. sapiens (human); GFP's
• Insert Size (bp): 2100
• Mutation: C418S mutation linker consists of 9 GGSGGS repeats
• Entrez Gene: ATOX1 (a.k.a. ATX1, HAH1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV promotor
• 3′ sequencing primer: CMV terminator

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry