Addgene - RasV12 in pENTR1A (w99-1) Plasmid Data

Plasmid 22252: RasV12 in pENTR1A (w99-1)

Gene/insert name:
HRAS G12V
Alt name:
RasV12
Insert size:
569
Species:
H. sapiens (human)
Entrez Gene:
HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Vector backbone:
pENTR1A
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
2466
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Eco RI
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
The RasV12 cDNA was obtained from Dr.Christian Beausejour.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22252" in your Materials and Methods section.