Addgene - pENTR/shp16 (w98-1) Plasmid Data

Plasmid 22258: pENTR/shp16 (w98-1)

Gene/insert name:
sh cyclin-dependent kinase inhibitor 2A
Alt name:
shp16
Insert size:
61
Species:
H. sapiens (human)
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
RNAi ; Entry vector
Backbone size w/o insert (bp):
2920
Cloning site 5':
Xho I
Site destroyed during cloning:
Yes
Cloning site 3':
Eco RI
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
shRNA obtained from Dr. Christian Beausejour.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22258" in your Materials and Methods section.