Addgene - pLenti CMV RasV12 Neo (w108-1) Plasmid Data

Plasmid 22259: pLenti CMV RasV12 Neo (w108-1)

Gene/insert name:
HRAS G12V
Alt name:
RasV12
Insert size:
569
Species:
H. sapiens (human)
Entrez Gene:
HRAS (C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Mutation:
G12V mutant
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8378
Cloning site 5':
Sal I
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
RasV12 cDNA obtained from Dr. Christian Beausejour.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22259" in your Materials and Methods section.