pLenti X2 Blast/shp16 (w112-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 7820
Vector typeLentiviral, RNAi
Growth in Bacteria
Growth instructionsStbl3, 37oC
Copy numberHigh Copy
Gene/Insert namesh cyclin-dependent kinase inhibitor 2A
Insert Size (bp)61
- Cloning method Restriction Enzyme
- 5′ cloning site Xho I (destroyed during cloning)
- 3′ cloning site Eco RI (not destroyed)
- 5′ sequencing primer U6
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byshp16 was obtained from Dr. Christian Beausejour.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti X2 Blast/shp16 (w112-1) was a gift from Eric Campeau (Addgene plasmid # 22261)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.