pQCXP CMV/TO RasV12 (w364-1)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22268||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7337
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Growth instructionsTOP10F', 37oC
Copy numberHigh Copy
Gene/Insert nameHRAS G12V
SpeciesH. sapiens (human)
Insert Size (bp)569
MutationGlycine 12 to Valine (G12V)
Entrez GeneHRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xba I (not destroyed)
- 5′ sequencing primer CMVforw
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byRasV12 cDNA was obtained from Dr. Christian Beausejour.
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pQCXP CMV/TO RasV12 (w364-1) was a gift from Eric Campeau (Addgene plasmid # 22268)
Generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.