Gene/insert name: HRAS G12V
Alt name: RasV12
Insert size: 569
Species:
H. sapiens (human)
Entrez Gene:
HRAS
(C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, K-RAS, N-RAS, RASH1)
Mutation: Glycine 12 to Valine (G12V)
Vector backbone:
pQCXIPSearch Vector Database )
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
7337
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw
List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
RasV12 cDNA was obtained from Dr. Christian Beausejour.
Sequence:
View sequences (3)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Addgene has sequenced a portion of this plasmid for verification.
Click here for the sequencing
result.
Please acknowledge the principal investigator and cite this article if you use
this plasmid in a publication. Also, please include the text "Addgene plasmid
22268" in your Materials and Methods section.
