Addgene - SV40 small + Large T in pENTR1A (w611-7) Plasmid Data

Plasmid 22297: SV40 small + Large T in pENTR1A (w611-7)

Gene/insert name:
SV40 small + Large T antigen
Alt name:
SV40 t+T Ag
Insert size:
2473
Species:
SV40
Vector backbone:
pENTR1A
(Search Vector Database)
Vector type:
Entry vector
Backbone size w/o insert (bp):
2398
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Bam HI
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
small + Large T Ag DNA was obtained from Dr. Stephane Gobeil.
Sequence: View sequences (3)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22297" in your Materials and Methods section.