pLenti CMV/TO SV40 small + Large T (w612-1)
Vector backbonep156RRL-sinPPT-CMV-GFP-PRE/Nhe I
- Backbone size w/o insert (bp) 6925
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
Growth instructionsStbl3, 37oC
Copy numberHigh Copy
Gene/Insert nameSV40 small + Large T antigen
Alt nameSV40 t+T Ag
Insert Size (bp)2472
- Cloning method Restriction Enzyme
- 5′ cloning site Bam HI (not destroyed)
- 3′ cloning site Xho I (not destroyed)
- 5′ sequencing primer CMVforw
- 3′ sequencing primer n/a (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySmall + large T antigen DNA from Dr. Stéphane Gobeil.
Terms and Licenses
The small and large T antigen inactivates the TP53, RB1 and PP2A genes.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti CMV/TO SV40 small + Large T (w612-1) was a gift from Eric Campeau (Addgene plasmid # 22298)
Map generated by Addgene from full sequence supplied by depositor.
Map uploaded by the depositor.