Addgene - pLenti CMV/TO SV40 small + Large T (w612-1) Plasmid Data

Plasmid 22298: pLenti CMV/TO SV40 small + Large T (w612-1)

Gene/insert name:
SV40 small + Large T antigen
Alt name:
SV40 t+T Ag
Insert size:
2472
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
6925
Cloning site 5':
Bam HI
Site destroyed during cloning:
No
Cloning site 3':
Xho I
Site destroyed during cloning:
No
5' sequencing primer:
CMVforw List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Small + large T antigen DNA from Dr. Stéphane Gobeil.
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

The small and large T antigen inactivates the TP53, RB1 and PP2A genes.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22298" in your Materials and Methods section.