GFP-PIPK1 gamma 90
(Plasmid #22299)

• Depositing Lab: Pietro De Camilli
• Publication: Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

Backbone

• Vector backbone: pEGFP-C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human phosphatidylinositol phosphate kinase type 1 gamma 90
• Alt name: PIPK1 gamma 90
• Alt name: KIAA0589
• Alt name: PtdInsPKI gamma-90
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• GenBank ID: NM_012398
• Entrez Gene: PIP5K1C (a.k.a. LCCS3, PIP5K-GAMMA, PIP5K1-gamma, PIP5Kgamma)
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: EGFP-N Sequencing Primere
• 3′ sequencing primer: EGFP-C Sequencing Primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr Nagase Kazusa DNA Research Institute
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry