Addgene - pFlag-CMV2-Brd4 (1-1362) Plasmid Data

Plasmid 22304: pFlag-CMV2-Brd4 (1-1362)

Gene/insert name:
BRD4
Insert size:
4100
Species:
H. sapiens (human)
Entrez Gene:
BRD4 (CAP, HUNK1, HUNKI, MCAP)
Fusion protein or tag:
Flag
Terminal:
N terminal on insert
Vector backbone:
pFLAG-CMV2
(Search Vector Database)
Backbone manufacturer:
Sigma
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4700
Cloning site 5':
HindIII
Site destroyed during cloning:
Unknown
Cloning site 3':
BamHI
Site destroyed during cloning:
Unknown
5' sequencing primer:
CMV-F List of Sequencing Primers
3' sequencing primer:
hGH-PA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (1)
Principal Investigator:
Eric Verdin
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription. Bisgrove et al (Proc Natl Acad Sci U S A. 2007 Aug 21. 104(34):13690-5. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22304" in your Materials and Methods section.