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pFlag-CMV2-Brd4 (1-1362)
(Plasmid #22304)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22304 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFLAG-CMV2
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRD4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4100
  • Entrez Gene
    BRD4 (a.k.a. CAP, HUNK1, HUNKI, MCAP)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFlag-CMV2-Brd4 (1-1362) was a gift from Eric Verdin (Addgene plasmid # 22304)
  • For your References section:

    Conserved P-TEFb-interacting domain of BRD4 inhibits HIV transcription. Bisgrove DA, Mahmoudi T, Henklein P, Verdin E. Proc Natl Acad Sci U S A. 2007 Aug 21. 104(34):13690-5. 10.1073/pnas.0705053104 PubMed 17690245