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Browse > David Bartel > Drinnenberg et al > plp
Plasmid 22310: plp

  • None

  • pYES2.1 modified
    (Search Vector Database)

  • Yeast Expression ; Integrating plasmid

  • 5857

  • PvuII

  • No

  • XbaI

  • No

  • GAL1 List of Sequencing Primers

  • CYC1

  • Ampicillin

  • DH5alpha

  • 37

  • Unknown

  • URA3

  • View sequences (2)

  • David Bartel

  • MTA

Comments: 

A MCS containing XhoI and EcoRI sites was cloned between the PvuII and XbaI sites of pYES2.1. The empty vector was created by replacing the hairpin of pIp-strongSC_GFP with XhoI and EagI sites

To make this plasmid integrating, the 2micron and f1 origins were replaced (using NheI and SpeI sites with sequence from S. castellii sc633:288301-289016 (amplified from genomic DNA with 5'-AAAAGCTAGCGATCCCTTATCAAATATGGTAC and 5'-AAAAACTAGTGTAGAATCCAGAGAATAGAATC).

Please note that Addgene's sequencing results differ from the full plasmid sequence from the depositing laboratory by a single nucleotide mismatch at bp# 3135 in the vector backbone. This mismatch is not known to affect plasmid function.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: RNAi in Budding Yeast. Drinnenberg et al (Science. 2009 Sep 10. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22310" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only