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pRS403-PGAL1-strongSC_GFP
(Plasmid #22316)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22316 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS403
  • Backbone size w/o insert (bp) 4456
  • Vector type
    Yeast Expression ; Integrating plasmid
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    SURE
  • Growth instructions
    Provided in SURE cells (Stratagene)
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-rad9-GFP
  • Insert Size (bp)
    623

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer CYC1
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GAL1 promoter, GFP silencing construct and CYC1 terminator fragment was cloned out of pYES2.1 and into pRS403.

The insert sequence can be found by clicking on the "View sequence" link. The rad9 intron loop is shown in upper case letters.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS403-PGAL1-strongSC_GFP was a gift from David Bartel (Addgene plasmid # 22316 ; http://n2t.net/addgene:22316 ; RRID:Addgene_22316)
  • For your References section:

    RNAi in Budding Yeast. Drinnenberg IA, Weinberg DE, Xie KT, Mower JP, Wolfe KH, Fink GR, Bartel DP. Science. 2009 Sep 10. ():. 10.1126/science.1176945 PubMed 19745116