|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||22465||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeInjection construct
Growth in Bacteria
Insert Size (bp)1500
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C, CMV-F, SP6 (Common Sequencing Primers)
pEntregfp3, p3Emcherry, and pCSDest2 were combined in a multisite reaction to create an expression clone where egfp (minus stop codon) is fused in frame with a C terminal mcherry tag
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSegfpcherry was a gift from Nathan Lawson (Addgene plasmid # 22465)
For your References section:Gateway compatible vectors for analysis of gene function in the zebrafish. Villefranc JA, Amigo J, Lawson ND. Dev Dyn. 2007 Nov . 236(11):3077-87. 10.1002/dvdy.21354 PubMed 17948311