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Hsp90 HA
(Plasmid #22487)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22487 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hsp90 HA
  • Alt name
    WT Hsp90beta HA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Hsp90 HA was a gift from William Sessa (Addgene plasmid # 22487 ; http://n2t.net/addgene:22487 ; RRID:Addgene_22487)
  • For your References section:

    Dynamic activation of endothelial nitric oxide synthase by Hsp90. Garcia-Cardena G, Fan R, Shah V, Sorrentino R, Cirino G, Papapetropoulos A, Sessa WC. Nature. 1998 Apr 23. 392(6678):821-4. 10.1038/33934 PubMed 9580552