Addgene - Ubc.IkBSR-Flag Pgk.Cre Plasmid Data

Plasmid 22503: Ubc.IkBSR-Flag Pgk.Cre

Gene/insert name:
IkBSR
Alt name:
IkappaBalpha super repressor
Alt name:
IkB-SR
Insert size:
969
Species:
M. musculus (mouse)
Fusion protein or tag:
Flag
Terminal:
C terminal on insert
Vector backbone:
UbC.Luciferase.PGK.Cre
(Search Vector Database)
Backbone manufacturer:
Available at Addgene (#20905)
Vector type:
Mammalian Expression, Lentiviral, Cre/Lox
Backbone size w/o insert (bp):
8609
Cloning site 5':
EcoRI
Site destroyed during cloning:
No
Cloning site 3':
NotI
Site destroyed during cloning:
No
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
MSCV rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
Unknown
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Tyler Jacks
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Mouse IkappaBalpha super repressor, Flag tagged (C-terminus). Cloned EcoRI-NotI in UbC.Luciferase.PGK.Cre vector in place of Luciferase: UBC promoter-Kozak-IkBaSR-Flag-STOP—PGK promoter-Cre.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22503" in your Materials and Methods section.