Addgene - pEF-ENTR-LUC (SN1) Plasmid Data

Plasmid 22524: pEF-ENTR-LUC (SN1)

Gene/insert name:
Firefly Luciferase
Insert size:
1652
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Luciferase
Backbone size w/o insert (bp):
4058
Cloning site 5':
Eco RV
Site destroyed during cloning:
Yes
Cloning site 3':
Xba I
Site destroyed during cloning:
No
5' sequencing primer:
T7forw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
Firefly luciferase was derived from pGL3 plasmid (Promega). EF1a promoter was derived from pTracer-Bsd plasmid (Invitrogen).
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA
Luciferase Limited Use Label License

Comments:

Vector made by Stefania Nicoli (University of Massachusetts Medical School).

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22524" in your Materials and Methods section.