Addgene - pENTR/pTER shATM #2 (730-2) Plasmid Data

Plasmid 22527: pENTR/pTER shATM #2 (730-2)

Gene/insert name:
sh Ataxia telangiectasia mutated
Alt name:
shATM
Insert size:
48
Vector backbone:
pENTR1A
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
RNAi ; Entry vector
Backbone size w/o insert (bp):
2571
Cloning site 5':
Bgl II
Site destroyed during cloning:
Yes
Cloning site 3':
Hind III
Site destroyed during cloning:
No
5' sequencing primer:
ENTRforw List of Sequencing Primers
3' sequencing primer:
ENTRrev
Bacterial resistance(s)
Kanamycin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
Growth instructions:
TOP10F', 37oC
High or low copy:
High Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

shATM #2 was used successfully in Rodier et al.(2009) Nat.Cell Biol. 11(8):973-979.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22527" in your Materials and Methods section.