Addgene - pLenti X2 Hygro/pTER shATM #2 (w639-1) Plasmid Data

Plasmid 22532: pLenti X2 Hygro/pTER shATM #2 (w639-1)

Gene/insert name:
sh Ataxia telangiectasia mutated
Alt name:
shATM
Insert size:
48
Vector backbone:
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I
(Search Vector Database)
Vector type:
Lentiviral, RNAi
Backbone size w/o insert (bp):
8459
Cloning site 5':
Bgl II
Site destroyed during cloning:
Yes
Cloning site 3':
Hind III
Site destroyed during cloning:
No
5' sequencing primer:
H1forw List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
Growth instructions:
Stbl3, 37oC
High or low copy:
High Copy
Selectable markers:
Hygromycin
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Eric Campeau
Terms and Licenses:
MTA

Comments:

shATM #2 was used successfully in Rodier et al.(2009) Nat.Cell Biol. 11(8):973-979.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22532" in your Materials and Methods section.

Price: $65.00

Available to academic and non-profits only

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This is commonly requested with

20958: pLenti X2 Hygro/pTER shLUC (w607-1)

17492: pLenti CMV TetR Blast (716-1)