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pAc-Neo-OVA
(Plasmid #22533)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 22533 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHBetaAPr-1-neo
  • Backbone manufacturer
    Kedes Lab
  • Backbone size w/o insert (bp) 10200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    OVA
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    1700
  • Entrez Gene
    OVAL (a.k.a. OVA, SERPINB14)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. B.W. O'Malley
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The OVA cDNA was removed from pOVA(230) (McReynolds et al., 1978) by digestion with EcoRI and subcloned into the polylinker of pUG-1. This provided convenient BglII and HindIII restriction sites adjacent to the ovalbumin cDNA for direct subcloning into the BamHI-HindIII site of the pHBetaAPr-1-neo express vector.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAc-Neo-OVA was a gift from Michael Bevan (Addgene plasmid # 22533 ; http://n2t.net/addgene:22533 ; RRID:Addgene_22533)
  • For your References section:

    Introduction of soluble protein into the class I pathway of antigen processing and presentation. Moore MW, Carbone FR, Bevan MJ. Cell. 1988 Sep 9. 54(6):777-85. 10.1016/S0092-8674(88)91043-4 PubMed 3261634