Addgene - pAc-Neo-OVA Plasmid Data

Plasmid 22533: pAc-Neo-OVA

Gene/insert name:
OVA
Insert size:
1700
Species:
G. gallus (chicken)
Entrez Gene:
SERPINB14 ()
Vector backbone:
pHBetaAPr-1-neo
(Search Vector Database)
Backbone manufacturer:
Kedes Lab
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
10200
Cloning site 5':
HindIII
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
unknown List of Sequencing Primers
3' sequencing primer:
SV40pA-R
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Unknown
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
Dr. B.W. O'Malley
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Michael Bevan
Terms and Licenses:
MTA

Comments:

The OVA cDNA was removed from pOVA(230) (McReynolds et al., 1978) by digestion with EcoRI and subcloned into the polylinker of pUG-1. This provided convenient BglII and HindIII restriction sites adjacent to the ovalbumin cDNA for direct subcloning into the BamHI-HindIII site of the pHBetaAPr-1-neo express vector.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Introduction of soluble protein into the class I pathway of antigen processing and presentation. Moore et al (Cell. 1988 Sep 9. 54(6):777-85. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22533" in your Materials and Methods section.