Addgene - Flag-HA-OTUB1 Plasmid Data

Plasmid 22551: Flag-HA-OTUB1

Gene/insert name:
OTUB1
Alt name:
OTU domain, ubiquitin aldehyde binding 1
Alt name:
FLJ20113, FLJ40710, HSPC263, MGC111158, MGC4584, OTB1, OTU1
Species:
H. sapiens (human)
GenBank ID:
BC007519.2
Entrez Gene:
OTUB1 (OTB1, OTU1, HSPC263, MGC4584, FLJ20113, FLJ40710, MGC111158)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Fusion protein or tag:
FLAG
Terminal:
N terminal on backbone
Mutation:
None
Vector backbone:
MSCV-N-Flag-HA-IRES-PURO
(Search Vector Database)
Backbone manufacturer:
Harper_Lab
Vector type:
Mammalian Expression, Retroviral
Backbone size w/o insert (bp):
6521
Cloning site 5':
GATEWAY_LR
Site destroyed during cloning:
Unknown
Cloning site 3':
GATEWAY_LR
Site destroyed during cloning:
Unknown
5' sequencing primer:
CAGCCCTCACTCCTTCTCTAGG List of Sequencing Primers
3' sequencing primer:
CAAGCGGCTTCGGCCAGTAAC
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
Marc_Vidal_Orfeome_collection
Sequence: View sequences (1)
Principal Investigator:
Wade Harper
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Defining the human deubiquitinating enzyme interaction landscape. Sowa et al (Cell. 2009 Jul 23. 138(2):389-403. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 22551" in your Materials and Methods section.

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